[Analysis and clinical application of preimplantation genetic testing for monogenic disorders in a case with Spinal muscular atrophy "2+0" genotype].

OBJECTIVE: To explore the clinical application of preimplantation genetic testing for monogenic disorders (PGT-M) in an unique case with Spinal muscular atrophy (SMA) type 2+0. METHODS: A special SMA family presented at the Third Affiliated Hospital of Guangzhou Medical University on October 19, 2020 was selected as the study subject. Multiple ligation-dependent probe amplification (MLPA) and molecular tagging linkage analysis were carried out to identify the SMN1 genotype of the couple and their fetus. Subsequently, next-generation sequencing (NGS), molecular tagging linkage analysis, and chromosomal microarray analysis were employed to determine the haplotypes and validate the result of PGT-M on the 11 embryos derived for the couple. RESULTS: The female partner was identified as a carrier of the rare SMN1[2+0] variant, and prenatal diagnosis confirmed the fetus to be affected by SMA. Ultimately, PGT-M has successfully selected four embryos free from the pathogenic SMN1 variants and X chromosome deletion. CONCLUSION: PGT-M can effectively prevent the transmission of rare genetic variants such as the SMA 2+0 subtype in the families. Above finding has provided guidance for genetic counseling and family planning for the couple.

Phage lysate can regulate the humification process of composting.

Phages play a crucial role in orchestrating top-down control within microbial communities, influencing the dynamics of the composting process. Despite this, the impact of phage-induced thermophilic bacterial lysis on humification remains ambiguous. This study investigates the effects of phage lysate, derived explicitly from Geobacillus subterraneus, on simulated composting, employing ultrahigh-resolution mass spectrometry and 16S rRNA sequencing techniques. The results show the significant role of phage lysate in expediting humus formation over 40 days. Notably, the rapid transformation of protein-like precursors released from phage-induced lysis of the host bacterium resulted in a 14.8 % increase in the proportion of lignins/CRAM-like molecules. Furthermore, the phage lysate orchestrated a succession in bacterial communities, leading to the enrichment of core microbes, exemplified by the prevalence of Geobacillus. Through network analysis, it was revealed that these enriched microbes exhibit a capacity to convert protein and lignin into essential building blocks such as amino acids and phenols. Subsequently, these components were polymerized into humus, aligning with the phenol-protein theory. These findings enhance our understanding of the intricate microbial interactions during composting and provide a scientific foundation for developing engineering-ready composting humification regulation technologies.

PH13 improves soybean shade traits and enhances yield for high-density planting at high latitudes.

Shading in combination with extended photoperiods can cause exaggerated stem elongation (ESE) in soybean, leading to lodging and reduced yields when planted at high-density in high-latitude regions. However, the genetic basis of plant height in adaptation to these regions remains unclear. Here, through a genome-wide association study, we identify a plant height regulating gene on chromosome 13 (PH13) encoding a WD40 protein with three main haplotypes in natural populations. We find that an insertion of a Ty1/Copia-like retrotransposon in the haplotype 3 leads to a truncated PH13H3 with reduced interaction with GmCOP1s, resulting in accumulation of STF1/2, and reduced plant height. In addition, PH13H3 allele has been strongly selected for genetic improvement at high latitudes. Deletion of both PH13 and its paralogue PHP can prevent shade-induced ESE and allow high-density planting. This study provides insights into the mechanism of shade-resistance and offers potential solutions for breeding high-yielding soybean cultivar for high-latitude regions.

Effect of noninvasive embryo viability testing versus conventional IVF on the live birth rate in IVF/ICSI patients: a study protocol for a double-blind, multicenter, randomized controlled trial.

BACKGROUND: Preimplantation genetic testing for aneuploidy (PGT-A) was demonstrated to be superior to conventional IVF in reducing the incidence of miscarriage and abnormal offspring after the first embryo transfer (ET). PGT-A requires several embryo trophectoderm cells, but its negative impacts on embryo development and long-term influence on the health conditions of conceived children have always been a concern. As an alternative, noninvasive PGT-A (niPGT-A) approaches using spent blastocyst culture medium (SBCM) achieved comparable accuracy with PGT-A in several pilot studies. The main objective of this study is to determine whether noninvasive embryo viability testing (niEVT) results in better clinical outcomes than conventional IVF after the first embryo transfer. Furthermore, we further investigated whether niEVT results in higher the live birth rate between women with advanced maternal age (AMA, > 35 years old) and young women or among patients for whom different fertilization protocols are adopted. METHODS: This study will be a double-blind, multicenter, randomized controlled trial (RCT) studying patients of different ages (20-43 years) undergoing different fertilization protocols (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]). We will enroll 1140 patients at eight reproductive medical centers over 24 months. Eligible patients should have at least two good-quality blastocysts (better than grade 4 CB). The primary outcome will be the live birth rate of the first embryo transfer (ET). Secondary outcomes will include the clinical pregnancy rate, ongoing pregnancy rate, miscarriage rate, cumulative live birth rate, ectopic pregnancy rate, and time to pregnancy. DISCUSSION: In this study, patients who undergo noninvasive embryo viability testing (niEVT) will be compared to women treated by conventional IVF. We will determine the effects on the pregnancy rate, miscarriage rate, and live birth rate and adverse events. We will also investigate whether there is any difference in clinical outcomes among patients with different ages and fertilization protocols (IVF/ICSI). This trial will provide clinical evidence of the effect of noninvasive embryo viability testing on the clinical outcomes of the first embryo transfer. TRIAL REGISTRATION: Chinese Clinical Trial Registry (ChiCTR) Identifier: ChiCTR2100051408. 9 September 2021.

Black soldier fly pretreatment promotes humification and phosphorus activation during food waste composting.

Black soldier fly (BSF) and thermophilic composting (TC) treatments are commonly adopted to manage food waste. In this study, 30 days of TC of food waste following seven days BSF pretreatment (BC) was compared to 37 days of TC of food waste (TC, the control). Fluorescence spectrum and 16S rRNA high-throughput sequencing analysis were used to compare the BC and TC treatments. Results showed that BC could decrease protein-like substances and increase humus substances more quickly, and that the humification index of compost products was 106.8% higher than that of TC, suggesting that the humification process was accelerated by BSF pretreatment resulting in a 21.6% shorter maturity time. Meanwhile, the concentrations of total and available phosphorus rose from 7.2 and 3.3 g kg-1 to 44.2 and 5.5 g kg-1, respectively, which were 90.5% and 118.8% higher in compost products from BC as compared to those in TC. Furthermore, BC had higher richness and diversity of humus synthesis and phosphate-solubilizing bacteria (PSB), with Nocardiopsis (53.8%) and Pseudomonas (47.0%) being the dominant PSB. Correlation analysis demonstrated that the introduction of BSF gut bacteria contributed to the effectiveness of related functional bacteria, resulting in a rapid humification process and phosphorus activation. Our findings advance understanding of the humification process and provide novel perspectives on food waste management.

Molecular Mechanisms and Clinical Phenotypes of GJB2 Missense Variants.

The GJB2 gene is the most common gene responsible for hearing loss (HL) worldwide, and missense variants are the most abundant type. GJB2 pathogenic missense variants cause nonsyndromic HL (autosomal recessive and dominant) and syndromic HL combined with skin diseases. However, the mechanism by which these different missense variants cause the different phenotypes is unknown. Over 2/3 of the GJB2 missense variants have yet to be functionally studied and are currently classified as variants of uncertain significance (VUS). Based on these functionally determined missense variants, we reviewed the clinical phenotypes and investigated the molecular mechanisms that affected hemichannel and gap junction functions, including connexin biosynthesis, trafficking, oligomerization into connexons, permeability, and interactions between other coexpressed connexins. We predict that all possible GJB2 missense variants will be described in the future by deep mutational scanning technology and optimizing computational models. Therefore, the mechanisms by which different missense variants cause different phenotypes will be fully elucidated.

Defining the sensitivity landscape of EGFR variants to tyrosine kinase inhibitors.

Tyrosine kinase inhibitor (TKI) is a standard treatment for patients with NSCLC harboring constitutively active epidermal growth factor receptor (EGFR) mutations. However, most rare EGFR mutations lack treatment regimens except for the well-studied ones. We constructed two EGFR variant libraries containing substitutions, deletions, or insertions using the saturation mutagenesis method. All the variants were located in the EGFR mutation hotspot (exons 18-21). The sensitivity of these variants to afatinib, erlotinib, gefitinib, icotinib, and osimertinib was systematically studied by determining their enrichment in massively parallel cytotoxicity assays using an endogenous EGFR-depleted cell line. A total of 3914 and 70,475 variants were detected in the constructed EGFR Substitution-Deletion (Sub-Del) and exon 20 Insertion (Ins) libraries. Of the 3914 Sub-Del variants, 221 proliferated fast in the control assay and were sensitive to EGFR-TKIs. For the 70,475 Ins variants, insertions at amino acid positions 770-774 were highly enriched in all 5 TKI cytotoxicity assays. Moreover, the top 5% of the enriched insertion variants included a glycine or serine insertion at high frequency. We present a comprehensive reference for the sensitivity of EGFR variants to five commonly used TKIs. The approach used here should be applicable to other genes and targeted drugs.

Study on Detection and Recognition of Traffic Lights Based on Improved YOLOv4.

To resolve the issues of a deep backbone network, a large model, slow reasoning speed on a mobile terminal, low detection accuracy for small targets and difficulties detecting and recognizing traffic lights in real time and accurately with YOLOv4, a traffic lights recognition method based on improved YOLOv4 is proposed. The lightweight ShuffleNetv2 network is utilized to replace the CSPDarkNet53 network of YOLOv4 to satisfy the requirements of a mobile terminal. The reformed k-means clustering algorithm is applied to generate anchor boxes for avoiding the sensitivity issue of outliers and initial values. A novel attention mechanism named CS2A is added to enhance the extraction capability of effective features. Multiple data augmentation methods are combined to improve the generalization ability of the model. Ultimately, the detection and recognition of traffic lights can be realized. The S2TLD dataset is selected for training and testing, and it can be proved that the recognition accuracy and model size are greatly optimized. Meanwhile, a self-made dataset is selected for training and testing. Compared with the conventional YOLOv4, the recognition accuracy of the proposed algorithm for traffic lights' state information increases by 1.79%, and the model size decreases by 81.97%. Appropriate scenes are selected for real-vehicle testing and the results demonstrate that the detection speed of the presented algorithm increases by 16%, and the recognition effect for small targets increases by 37% in comparison with conventional YOLOv4.

Identification of QTLs and joint QTL segments of leaflet traits at different canopy layers in an interspecific RIL population of soybean.

KEY MESSAGE: A leaflet trait on different canopy layers may have different QTLs; leaflet trait QTLs may cluster to form joint QTL segments; all canopy layer QTLs form a complete QTL system for a leaflet trait. As the main part of the plant canopy structure, leaf/leaflet size and shape affect the plant architecture and yield. To explore the leaflet trait QTL system, a population composed of 199 recombinant inbred lines derived from Changling (annual wild, narrow leaflet) and Yiqianli (landrace, broad leaflet) with their parents was tested for leaflet length (LL), width (LW) and length to width (LLW). The population was genotyped with specific-locus amplified fragment sequencing (SLAF-seq) and applied for linkage mapping of the leaflet traits. The results showed that the leaflet traits varied greatly even within a plant, which supported a stratified leaflet sampling strategy to evaluate these traits at top, middle and bottom canopy layers. Altogether, 13 LL, 10 LW and 9 LLW in a total of 32 plus 3 duplicated QTLs were identified, in which, 17 QTLs were new ones, and 48.6%, 28.6% and 22.8% of QTLs were from the top, middle and bottom layers, respectively, indicating the genetic importance of the top layer leaves. Since a leaflet trait may have layer-specific QTLs, all layer QTLs form a complete QTL system. Five QTL clusters each with their QTL supporting intervals overlapped were designated as joint QTL segments (JQSs). In JQS-16, with its linkage map further validated using PCR markers, two QTLs, qLW-16-1 and qLLW-16-1 of the top layer leaflet, were identified six QTL·times. Six candidate genes were predicted, with Glyma.16G127900 as the most potential one for LW and LLW. Three PCR markers, Gm16PAV0653, BARCSOYSSR_16_0796 and YC-16-3, were suggested for marker-assisted selection for LW and LLW in JQS-16.

Differentiated strategies of animal-derived and plant-derived biochar to reduce nitrogen loss during paper mill sludge composting.

This work aimed to reveal the differences of nitrogen (N) transformation between animal-derived and plant-derived biochar during paper mill sludge composting. Three treatments were established, including CK (no biochar), ABC (animal-derived biochar), and PBC (plant-derived biochar). Results showed that N loss was reduced by 24.43% and 35.50% in ABC and PBC, respectively, compared with CK. Moreover, the contents of acid-insoluble N (AIN) in ABC and bioavailable organic N (BON) in PBC were 6.180 g/kg and 9.269 g/kg higher than in CK (2.602 g/kg and 8.988 g/kg). The protease activity and bacterial abundance associated with the generation of humic N-containing precursors increased in ABC. Low urease activity and a more complex bacterial N-cycling network were found in PBC. Structural equation model confirmed that AIN formation and BON retention were the dominant strategies for animal-derived and plant-derived biochar, respectively. The findings provided multiple pathways to produce N-enriched compost products.

Linking Microbial Community Succession With Substance Transformation in a Thermophilic Ectopic Fermentation System.

Ectopic fermentation system (EFS) is an effective technology for treating mass livestock manure. However, the associations between microbial communities and substance transformation remain controversial. This study aimed to investigate chicken manure EFS lasting 170 days using 16S rRNA sequencing and electrochemical, spectroscopic, and chromatographic analyses. The results showed a noticeable transformation of protein-like substances into humus-like substances. Meanwhile, the electron-accepting capacity increased persistently, effectively reflecting the humification of organic substances. The contents of phenols that promoted electron transfer continued to increase from 2.80 to 6.00%, which could be used as a maturity indicator for EFS. During the heating period, the dominant microbial communities were Chloroflexi and Proteobacteria, whereas thermotolerant bacteria Cyanobacteria and Planctomycetes were significantly enriched from 1.64 to 50.15% during the continuous thermophilic period of EFS. The correlation analysis manifested that these thermotolerant bacteria were the major functional bacteria for the formation of phenols and the key to driving the humification of organic substances. This study provides insights into understanding the humification mechanisms and implementing regulatory strategies in EFS.

The intervention of classical and molecular breeding approaches to enhance flooding stress tolerance in soybean - An review.

Abiotic stresses and climate changes cause severe loss of yield and quality of crops and reduce the production area worldwide. Flooding stress curtails soybean growth, yield, and quality and ultimately threatens the global food supply chain. Flooding tolerance is a multigenic trait. Tremendous research in molecular breeding explored the potential genomic regions governing flood tolerance in soybean. The most robust way to develop flooding tolerance in soybean is by using molecular methods, including quantitative trait loci (QTL) mapping, identification of transcriptomes, transcription factor analysis, CRISPR/Cas9, and to some extent, genome-wide association studies (GWAS), and multi-omics techniques. These powerful molecular tools have deepened our knowledge about the molecular mechanism of flooding stress tolerance. Besides all this, using conventional breeding methods (hybridization, introduction, and backcrossing) and other agronomic practices is also helpful in combating the rising flooding threats to the soybean crop. The current review aims to summarize recent advancements in breeding flood-tolerant soybean, mainly by using molecular and conventional tools and their prospects. This updated picture will be a treasure trove for future researchers to comprehend the foundation of flooding tolerance in soybean and cover the given research gaps to develop tolerant soybean cultivars able to sustain growth under extreme climatic changes.

[Quercetin for the treatment of prostate cancer: Progress in studies].

Prostate cancer (PCa) is a common urinary malignancy, and advanced PCa has a poor prognosis and a high mortality. Drug therapies currently available for this malignancy often cause serious adverse reactions, and therefore new drugs with fewer adverse effects or the potential to reduce the adverse effects of traditional chemotherapeutic drugs are badly needed for the management of PCa. Quercetin, as a natural flavonoid, has been extensively studied in recent years for its anti-cancer effects, as in cell signal transduction, apoptosis promotion, anti-proliferation and -oxidation, and growth inhibition. In fact, quercetin has a variety of biological effects and can inhibit various enzymes involved in cell proliferation and signal transduction pathways. Besides, quercetin is also reported to have potential synergistic effects when used in combination with radiotherapy or chemotherapeutic drugs. This review summarizes the advances in the treatment of PCa with quercetin, focusing on its effects of promoting the apoptosis, inhibiting the proliferation and reducing the invasiveness and migration of tumor cells, and reversing drug resistance, aiming to provide a new theoretical basis and some new ideas for the studies of the treatment of PCa.

CCDC114, DNAI2 and TOP2A involves in the effects of tibolone treatment on postmenopausal endometrium.

BACKGROUND: This study aimed to explore the molecular mechanisms of tibolone treatment in postmenopausal women. METHODS: The gene set enrichment profile, GSE12446, which includes 9 human endometrial samples from postmenopausal women treated with tibolone (tibolone group) and 9 control samples (control group), was downloaded from GEO database for analysis. Differentially expressed genes (DEGs) in tibolone vs. control groups were identified and then used for function and pathway enrichment analysis. Protein-protein interaction (PPI) network and module analyses were also performed. Finally, drug-target interaction was predicted for genes in modules, and then were validated in Pubmed. RESULTS: A total of 238 up-regulated DEGs and 72 down-regulated DEGs were identified. These DEGs were mainly enriched in various biological processed and pathways, such as cilium movement (e.g., CCDC114 and DNAI2), calcium ion homeostasis, regulation of hormone levels and complement/coagulation cascades. PPI network contained 368 interactions and 166 genes, of which IGF1, DNALI1, CCDC114, TOP2A, DNAH5 and DNAI2 were the hue genes. A total of 96 drug-gene interactions were obtained, including 94 drugs and eight genes. TOP2A and HTR2B were found to be targets of 28 drugs and 38 drugs, respectively. Among the 94 obtained drugs, only 12 drugs were reported in studies, of which 7 drugs (e.g., epirubicin) were found to target TOP2A. CONCLUSIONS: CCDC114 and DNAI2 might play important roles in tibolone-treated postmenopausal women via cilium movement function. TOP2A might be a crucial target of tibolone in endometrium of postmenopausal women.

Hyperthermophilic composting significantly decreases methane emissions: Insights into the microbial mechanism.

Methane (CH4) emissions from thermophilic composting (TC) are a substantial contributor to climate change. Hyperthermophilic composting (HTC) can influence CH4-related microbial communities at temperatures up to 80 °C, and thus impact the CH4 emissions during composting. This work investigated CH4 emissions in sludge-derived HTC, and explored microbial community succession with quantitative PCR and high-throughput sequencing. Results demonstrated that HTC decreased CH4 emissions by 52.5% compared with TC. In HTC, the CH4 production potential and CH4 oxidation potential were nearly 40% and 64.1% lower than that of TC, respectively. There was a reduction in the quantity of mcrA (3.7 × 108 to 0 g-1 TS) in HTC, which was more significant than the reduction in pmoA (2.0 × 105 to 2.1 × 104 g-1 TS), and thus lead to reduce CH4 emissions. It was found that the abundance of most methanogens and methanotrophs was inhibited in the hyperthermal environment, with a decline in Methanosarcina, Methanosaeta and Methanobrevibacter potentially being responsible for reducing the CH4 emissions in HTC. This work provides important insight into mitigating CH4 emissions in composting.

Protein-derived structures determines the redox capacity of humic acids formed during hyperthermophilic composting.

Humic acid (HA) in compost has received widespread attention for its high redox activity, which can mediate the degradation of organic pollution and the passivation of heavy metals in the environment. Hyperthermophilic composting (HTC) can accelerate HA formation. However, few studies have examined whether and how the structures of different organics affect the formation of the HA and HA redox structure at the molecular level in HTC. Detailed molecular information and the redox capacity (electron transfer capacity, ETC) of HA in HTC and thermophilic composting (TC) were characterized using pyrolysis gas chromatography/mass spectrometry and the electrochemical method, respectively. HTC promoted the formation of redox structure, leading to the improvement of the ETC of HA. Aromatics and N-containing compounds were mainly derived from protein components, and the rate at which they were transferred into HA was accelerated in HTC, while the relative abundance of lipids decreased. Partial least squares regression and correlation analysis demonstrated that protein-derived compounds were the key factor determining the HA redox capacity. Finally, partial least squares path modeling suggested that the influence mechanism of protein-derived structures on HA redox capacity might differ in HTC and TC. HTC may promote the relative abundance of N-containing components into the C-skeleton and accelerate the accumulation of the aromatic products, thereby improve the HA redox capacity. These findings provided new insight into how the redox capacity of the HA in compost could be improved and how compost products could be prepared for use in environmental remediation.

Alkali lignin and sodium lignosulfonate additives promote the formation of humic substances during paper mill sludge composting.

Alkali lignin (AL) and sodium lignosulfonate (SLS) are by-products of the papermaking industry and could influence composting processes due to their rich aromatic structures. In this study, the roles of AL and SLS additives in the formation of humic substances (HS) during paper mill sludge composting were investigated. Results showed that HS content and degree of polymerization of the final products in AL (44.42 mg·g-1 and 0.70, respectively) and SLS (45.87 mg·g-1 and 1.14, respectively) treatments were appreciably higher than those of the control sample (34.36 mg·g-1 and 0.67). Excitation-emission matrix-parallel factor coupled with two-dimensional FT-IR correlation spectroscopy analysis suggested that AL and SLS additives could speed the transformation of quinone-like substances by increasing the amounts of low molecular weight lignin depolymerized products, which led to higher HS concentrations. This work provided a way of promoting HS formation and the comprehensive utilization of papermaking wastes.

Comparison of molecular transformation of dissolved organic matter in vermicomposting and thermophilic composting by ESI-FT-ICR-MS.

The aim of this study was to investigate the effects of vermicomposting (VC) and thermophilic composting (TC) on the molecular transformation of dissolved organic matter (DOM). Here, the DOM after VC and TC (DOMv and DOMt, respectively) was characterized using electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS). The results indicated that VC could improve the preservation of nitrogen and the humification of DOM compared with TC. Concurrently, VC facilitated the formation of highly oxidized molecules (O/C = 0.4-0.9) by enhancing the oxidation. The aromatized molecules in each component were more easily generated during VC, especially N-containing aromatized molecules (39.4-58.0%), thereby improving the quality of compost products. Furthermore, this study found that VC could reduce the anaerobic microsites in pile, thus increasing nominal oxidation state of carbon (NOSC) of organic matter and promoting the decomposition of high-energy substrates (mainly lipids, NOSC = - 1.7~- 1.3). These findings provided new molecular insights that VC can significantly improve the oxidation of organic matter and the preservation of nitrogen. Graphical abstract.

Evaluation of left ventricular systolic function using synchronized analysis of heart sounds and the electrocardiogram.

BACKGROUND: Heart failure is a major health concern and often requires echocardiography to confirm the diagnosis. We introduce a new method that uses a wearable heart sound and electrocardiogram (ECG) device that can be used in the outpatient setting. OBJECTIVE: The purpose of this study was to determine the value of synchronized analysis of heart sounds and ECG in identifying patients with depressed left ventricular ejection fraction (dLVEF) <50%. METHODS: One hundred eighty-nine patients (76 with dLVEF; 113 with normal ejection fraction) were enrolled. All were admitted to the hospital because of dyspnea or chest discomfort. N-Terminal pro-B-type natriuretic peptide (NT-proBNP) was measured in all patients. LVEF was determined by echocardiography. Heart sound and ECG signals were simultaneously recorded using the wearable synchronized phonocardiogram and ECG device. Heart sound and ECG signals were automatically analyzed using wavelet analysis and utilized to determine electromechanical activation time (EMAT), EMAT/RR, S1-S2 time, and S1-S2/RR. RESULTS: EMAT in the dLVEF group was significantly higher than that in the control group (159.82 ± 83 ms vs 91.58 ± 28 ms). Pearson correlation test showed a negative correlation between EMAT and LVEF (r = -0.449; P <.001). Receiver operating characteristic curve analysis demonstrated that the sensitivity and specificity of EMAT ≥104 ms for the diagnosis of EF <50% were 92.1% and 92%, respectively. Patients with intermediate NT-proBNP values were identified as dLVEF by EMAT ≥104 ms, with sensitivity of 93.5% and specificity of 92.8%. CONCLUSION: The heart sound and ECG signal index EMAT contributes to the diagnosis of EF <50% and is especially helpful in patients with an inconclusive NT-proBNP value.

A Shared Regulatory Element Controls the Initiation of Tcf7 Expression During Early T Cell and Innate Lymphoid Cell Developments.

The transcription factor TCF-1 (encoded by Tcf7) plays critical roles in several lineages of hematopoietic cells. In this study, we examined the molecular basis for Tcf7 regulation in T cells, innate lymphoid cells, and migratory conventional dendritic cells that we find express Tcf7. We identified a 1 kb regulatory element crucial for the initiation of Tcf7 expression in T cells and innate lymphoid cells, but dispensable for Tcf7 expression in Tcf7-expressing dendritic cells. Within this region, we identified a Notch binding site important for the initiation of Tcf7 expression in T cells but not in innate lymphoid cells. Our work establishes that the same regulatory element is used by distinct transcriptional controllers to initiate Tcf7 expression in T cells and ILCs.